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TABLE 2

BONE MARROW BIOPSY
IMMUNOHISTOCHEMISTRY ON PARAFFIN SECTIONS

(Table not exhaustive !)

CD45    Leukocyte Common Antigen (LCA)
CD138/syndecan-1 Plasma cells
Immunoglobulins A, G, M, Kappa, Lambda
   
CD20 (L26) Pan B
CD45RA (4KB5) Pan B
CD79a (JCB117) Pan B
DBB42 Pan B, a few T-cells
CD72/DBA44 Hairy cells, a few B-cells
   
CD3 Pan T
CD45R0 (UCHL1) Pan T, a few granulocytic, monocytic and B-cells
CD8 (C8/144B) T8
CD1a (NA1/34) Langerhans histiocytosis cells, immature T cells
CD57 (Leu7) T cells, NK cell subsets
CD56 (Leu19) NK cells, cytotoxic T cell subsets, neuroectodermal cells
Glycophorine A Erythroid cell line
Myeloperoxydase Granulocytic cell line
Elastase (NP57) Granulocytic cell line
CD68 (KP1) Myelomonocytic cells
CD68 (PGM1) Histiocytes and monocytes
AA1 (Tryptase) Mast cells
Factor VIII (VW) Megacaryocytes, endothelium
CD61 (Y2/51)  ""
UEA (Ulex)  ""
   
CD30 (BerH2) Activated lymphoid cells, Hodgkin's cells, ALCL cells
CD15 (LeuM1) Granulocytes, Hodgkin's cells
   
CD34 (QBEND10) Immature lymphoid and myeloid cells or stem cells, endothelium
TdT Immature lymphoid cells, lymphoblastic ML, ALL, rarely undifferentiated AML
EMA Epithelial Membrane Antigen, plasma cells, ALCL cells
Cytokeratin (MNF116) Epithelial cells

Abbr.:
NK, Natural Killer
ML, Malignant Lymphoma
ALCL, Anaplastic Large Cell Lymphoma
TdT, Terminal Desoxynucleotidyl Transferase
ALL, Acute Lymphoblastic Leukaemia
AML, Acute Myeloid Leukaemia

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